PCR Reaction Components

This is a generic mix; check the literature that came with whatever Taq polymerase you are using:

For 25 ul reaction:

10X Buffer                              2.5 ul
MgCl (25 mM stock)                  2 ul
dNTPs (5 mM stock)                 1 ul
Primer A (0.25 pM stock)         1 ul
Primer B (0.25 pM stock)         1 ul
Taq polymerase                   0.15 ul
DNA                             10 - 500 ng
H20                             add to 25 ul total